100mM Potassium Acetate Solution

100mM Potassium Acetate Solution – Laboratory-Grade Buffer for Analytical & Molecular Biology Applications

SHANXI ZHAOYI CHEMICAL CO., LTD. has been making acetate reagents since 1988. That adds up to more than 35 years of production experience. Our standardized plant covers 27,000 m². Each year we turn out 150,000 tons of acetate products, and lab chemical reagents ship to customers worldwide on a steady basis. Our100mM Potassium Acetate Solution (CAS 127-08-2) is a clear, colorless liquid buffer reagent of premium grade, mixed to a standard strength of 100 mmol/L. It follows strict analytical chemistry industry standards. Because the solution arrives ready to use, errors from preparing buffer by hand drop away. ISO, KOSHER and HALAL certifications are all in place, so batch after batch stays consistent. That makes it a good fit for high-precision biochemical work and molecular biology research. Packaging can be customized from 500mL to 5L, which covers the different needs of most labs.

Key Features & Benefits

Concentration on our this product is accurate. Its pH value holds steady, and it works well with a wide range of experiments. Several common headaches in lab reagent prep and testing are solved by this product:

Analytical Precision: Strict closed-loop conditions govern both production and testing of this reagent. Molarity accuracy stays within ±2%, which is far tighter than what conventional lab preparation reaches. The formula is pure and transparent. As a result, no spectral interference shows up in UV-Vis spectroscopy detection or in enzymatic reaction experiments. Experimental data comes out accurate and repeatable.

Application Versatility: The grade fits DNA/RNA precipitation work. Protein electrophoresis buffer prep is another use, and so is setting up an HPLC mobile phase. Want a different pH? We adjust it free of charge anywhere between 4.5 and 7.5. That saves labs the slow job of debugging a second time, and the solution matches their protocol straight away.

Strict Quality Assurance: Every batch is checked closely before it leaves us. The main things we look for are heavy metals and impurity contamination. Reagents have been our team's work for decades. Year after year, university labs, research institutes, and pharma R&D firms get a steady supply they can count on.

Technical Specifications

How It Works

What you have here is a weak electrolyte buffer, and it holds its stability well. This product keeps the ionic strength of water-based solutions in check and holds it steady. Precision experiments depend on that steady behaviour. The chemistry behind it works like this:

Nucleic Acid Purification Mechanism: During a standard DNA or RNA extraction, potassium ions clump SDS-protein complexes and cell debris together. With nucleic acids, acetate anions do the reverse job. Those molecules stay in solution instead of dropping out and getting lost. Recovery rate goes up, and sample purity rises with it.

Chromatography Compatibility: UV light passes straight through this solution below 210 nm. It hardly reacts with other chemicals either. Metal-sensitive enzymes are not dragged into a chelation reaction with it, so that kind of interference never reaches your assay. Protein purification runs are a good fit. So is enzyme kinetic analysis.

Stable Buffering Performance: Its dissociation shifts only mildly with temperature. Buffering therefore holds steady across the temperature range a normal lab works in. Baseline drift in analytical detection drops when you compare it with phosphate and chloride buffers. Results repeat well from one run to the next.

Applications

Scientific research institutions around the world know this lab-grade buffer reagent. It covers the core scenarios, from molecular biology research to analytical chemistry detection. Biochemical assay experiments fall under it as well:

Molecular Biology Research: It works for plasmid DNA purification using 100mM Potassium Acetate Solution by the alkaline lysis method. Genomic DNA and other impurities come out as a precipitate. What stays in the solution is high-purity plasmid DNA. Bacterial transformation succeeds more often as a result. Purity goes up too.

Analytical Chemistry: Plenty of labs reach for it when they prepare mobile phases for HPLC. Ion chromatography is another common use. Unlike non-volatile salt reagents, it volatilizes well under mass spectrometry conditions. Background noise drops, and ionization detection comes out cleaner. A stable pH keeps retention time steady in gradient elution tests.

Biochemical Assays: The acetate components do not block enzyme activity. Steady ionic strength is available for experiments that detect enzyme activity. Protein samples keep a stable shape during dialysis treatment. Long-term sample storage also gets an effective cryoprotectant from this solution.

100mM Potassium Acetate Solution vs Other Products

Certifications & Compliance

Our production base runs under the ISO 9001:2015 quality management system, and we stick to it. Control checks cover the whole process. KOSHER and HALAL certification standards are met by this laboratory-grade reagent. Traceability management for food chemicals is supported too. Qualified, audited suppliers are the only source we use for raw materials. USP and EP pharmacopoeia specs are fully met by those materials. Every batch of goods ships with an official Certificate of Analysis (CoA). Batch concentration, pH value, and impurity indicators are all written down in that CoA. Those records help customers with experimental verification and batch traceability.

Packaging Options

We supply packaging built for laboratory-grade work. It keeps reagents from getting contaminated and helps them stay stable over time:

Standard Lab Packaging: PTFE-lined leak-proof caps come on our 500mL amber glass bottles. Everyday conventional experiments can use 1L HDPE plastic bottles. High-throughput lab work calls for 5L large-capacity carboys.

Customized Packaging: OEM volume packaging is available from us. We also supply finished products at sterile filtration grade. Special experimental scenarios get the format they need. The same applies to customers who have worked with us for years.

Each packaging container carries UN non-hazardous goods certification. So the containers line up with international shipping standards. Cross-border transport stays safe. Pollution is not an issue either.

Manufacturing Process

Finished reagents hold up with very high stability. Two things make that happen: high-purity raw materials and a standardized production process. The raw material is 99.5% high-purity potassium acetate crystals. Glacial acetic acid and potassium hydroxide are neutralized to synthesize those crystals.

Dissolving takes place inside a stainless steel reactor. Dilution happens in that same vessel. Nitrogen protection and triple-distilled water fill it. Carbonate impurity contamination stays out this way. Molarity gets checked in real time by online conductivity sensors. Dilution accuracy holds within ±0.5%.

pH is driven to the exact point needed with glacial acetic acid or potassium hydroxide. Care matters at this step. Next comes a 0.22μm precision filter, which pulls out tiny particles from the solution. Finished products are stored in a constant temperature warehouse kept at 18-22°C. Concentration deviation stays away. Reagent performance holds steady.

Why Choose Us

Rich Industry Experience: Acetate reagent production, including 100mM Potassium Acetate Solution, has been our work for over 35 years. Out of those years grew long-term stable cooperative relationships with scientific research institutions in Europe, America, Asia and other regions. Analytical reagent formula optimization and experimental matching are things our professional R&D team knows inside out.

Stable Supply Capacity: We turn out 150,000 tons a year. Plenty of safety stock sits in our warehouse. A standard order ships in 7 days on the fast cycle. That way a reagent shortage never holds up your research.

Professional Technical Support: Experiment design help and technical advice cost you nothing. Our team also answers questions about your protocol. Something going wrong at the bench? We have seen the same problem at many customer sites and can point you to a fix. The same goes for taking a formula up to production scale.

FAQ

Q: Can you customize specific pH values for special experiments?

A: A1: Yes. We support free pH customization in the range of 4.5-7.5 for orders over 10L. You only need to specify the target pH value when placing an order to get tailored reagent products.

Q: Is your standard solution RNase/DNase-free?

A: A2: The regular grade does not carry nuclease-free certification. Use our professional molecular biology grade for sensitive nucleic acid work and molecular experiments. It is sterile-filtered and tested for nucleases.

Q: After the package is opened, how long can the product still be used?

A: A3: After you open it, screw the cap back on tight. Store the bottle at 4°C. Over 6 months, the reagent concentration changes by less than 1% under standard storage conditions. That's enough for typical experiments.

Q: Are there price breaks if I buy in large quantities?

A: A4: Yes. We have exclusive preferential pricing for orders above 50L. You can contact our sales team to obtain customized bulk quotation and delivery schemes.

Q: Can I get free samples for experimental verification?

A: A5: We provide 100mL free samples for qualified scientific research institutions and laboratory customers. International orders only need to bear the corresponding freight cost.

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If you are looking for high-precision 100mM Potassium Acetate Solution and molecular biology experiments, sxzy@sxzhaoyi.com.